Ampholytes 3-10 Isodalt (Servalyt 3-10 Isodalt)

Carrier Ampholytes 3-10 Isodalt

SERVALYT™ 3-10 Isodalt ampholytes are suited for 2D-gel electrophoresis to identify isotypes. Ampholytes are supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. 

Benefits:

• Available Two Sizes: 10 ml & 25ml
• High Resolution
• Widest Range Available
• Fast Staining and Clear Background
• Ready to Use or Blending
• 3 year shelf life

Servalyt ampholyte instructions

Comparison of different brands of carrier ampholytes

The benefits and applications of using Isodalt 2DE in IEF and 2DE include:

Enhanced protein separation: Isodalt 2DE is formulated to provide an optimized pH gradient across a wide range (typically pH 3-10) for improved separation of proteins based on their isoelectric points (pI). This allows for increased resolution and better discrimination of proteins with different pI values.

Increased protein detection: By achieving better separation and focusing of proteins in the first dimension, Isodalt 2DE enhances the detection sensitivity of proteins in the subsequent second dimension (SDS-PAGE). This leads to improved visualization and identification of proteins of interest.

Comprehensive protein analysis: 2DE using Isodalt 2DE allows for the separation and visualization of thousands of proteins in a single gel. This comprehensive analysis provides valuable information on protein expression patterns, post-translational modifications, and protein isoforms, enabling deeper insights into cellular processes and disease mechanisms.

Biomarker discovery: The combination of IEF with SDS-PAGE using Isodalt 2DE is widely used in biomarker discovery studies. By comparing protein expression profiles between different samples, such as healthy and diseased tissues, potential biomarkers associated with specific conditions or diseases can be identified.

Proteomics research: Isodalt 2DE is a common tool in proteomics research, allowing for the systematic analysis of protein expression patterns in various biological samples. It provides a means to compare protein profiles between different experimental conditions or study dynamic changes in protein expression over time.

Disease research and diagnostics: Isodalt 2DE has been extensively used in disease-related research to study changes in protein expression associated with various disorders, such as cancer, neurological diseases, and cardiovascular diseases. It can also contribute to the development of diagnostic markers and potential therapeutic targets. 

In summary, Isodalt 2DE, as an ampholyte mixture used in the first dimension of 2DE, offers enhanced protein separation, increased protein detection, comprehensive protein analysis, biomarker discovery capabilities, and finds applications in proteomics research and disease-related studies.