Ampholytes 4-5 (Servalyt 4-5)

Carrier ampholytes 4-5

SERVALYT™ 4-5 carrier ampholytes are useful in identifying acidic isotypes in purified protein samples such as monoclonal antibodies or other biosimilar protein products.

Supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. Excellent for high resolution of acidic protein in isoelectric focusing (IEF), capillary isoelectric focusing (cIEF) and free flow electrophoresis applications.

Benefits:

• Available Two Sizes: 10 ml & 25ml
• High Resolution
• Widest Range Available
• Fast Staining and Clear Background
• Ready to Use or Blending
• 3 year shelf life

Instructions for Servalyt carrier ampholytes

Comparison of different brands of carrier ampholytes

Using an ampholyte pH 4-5 in isoelectric focusing (IEF) offers several applications and benefits, including:

Specific separation of proteins with intermediate pI values: The pH range of 4-5 is suitable for the specific separation of proteins with intermediate isoelectric points (pI). It allows for focused separation and resolution of proteins that have pI values within this range, which may include proteins with slightly acidic or slightly basic characteristics.

High resolution for proteins with intermediate pI: The use of an ampholyte pH 4-5 enables high-resolution separation of proteins with intermediate pI values. The narrow pH gradient created by the ampholytes allows for precise migration and focusing of these proteins at their respective pI positions. This results in sharp and well-resolved protein bands, facilitating accurate identification and characterization.

Minimization of interference: By using an ampholyte pH 4-5, the focusing range is limited to the intermediate pH region, minimizing potential interference from proteins with higher or lower pI values. This is particularly beneficial when dealing with complex samples containing a mixture of proteins with varying pI values, allowing for selective separation and analysis of the proteins with intermediate pI values.

Improved sensitivity for proteins with intermediate pI: The focused separation of proteins with intermediate pI values achieved with an ampholyte pH 4-5 can enhance the sensitivity of detection methods. When analyzing low abundance proteins with intermediate pI values, the reduced complexity of the pH range can lead to improved detection limits and a higher signal-to-noise ratio.

Simplified experimental setup: The use of a narrow pH range such as 4-5 simplifies the experimental setup for IEF. The preparation of the ampholyte mixture and the establishment of the pH gradient become more straightforward and manageable. This saves time and reduces potential sources of experimental variability.

Research applications: Using an ampholyte pH 4-5 in IEF finds relevance in research areas where proteins with intermediate pI values are of interest. It provides a specialized tool for investigating and separating proteins with specific characteristics within this pH range.

In summary, using an ampholyte pH 4-5 in IEF offers specific separation and high resolution for proteins with intermediate pI values, minimizes interference from other proteins, increases sensitivity, simplifies experimental setup, and finds application in research areas focused on proteins with intermediate pI values.