Servalyts for isoelectric focusing
Ampholytes or carrier ampholytes are water soluble amphoteric molecules that can perform as either a basic or acid molecule depending on the pH of the buffer it is in. They are most frequently used in applications such as isoelectric focusing, 2D gel electrophoresis and capillary isoelectric focusing. These techniques are often employed to discover isotypes of proteins; particularly monoclonal antibodies or biosimilar proteins.
Servalyts provide a broad selection of pH ranges for Isoelectric Focusing. This wide pH range includes ampholytes 2-11 and as narrow as ampholyte 4-5 for single point isoelectric focusing. The have been independently evaluated by Agilent for use in Capillary Electrophoresis and found to have greater resolution that Pharmalyte ampholytes and HR AESlyte ampholytes.
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