Ampholytes 5-6 (Servalyt 5-6) 

Carrier ampholytes pH 5-6

Applications: Isoelectric focusing, capillary electrophoresis, free-flow electrophoresis

SERVALYT 5-6 carrier ampholytes are useful in identifying acidic isotypes in purified protein samples such as monoclonal antibodies or other biosimilar protein products.

Supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. Excellent for high resolution of acidic protein in isoelectric focusing (IEF), capillary isoelectric focusing (cIEF) and free flow electrophoresis applications.

Benefits:

• Available Two Sizes: 10 ml & 25ml
• High Resolution
• Widest Range Available
• Fast Staining and Clear Background
• Ready to Use or Blending
• 3 year shelf life

Comparison of different brands of carrier ampholytes

Using an ampholyte pH 5-6 in isoelectric focusing (IEF) offers several applications and benefits, including:

Focused separation of proteins with specific pI values: The pH range of 5-6 allows for the focused separation of proteins with isoelectric points (pI) within this range. It is particularly suitable for proteins with slightly acidic to neutral characteristics. By providing a narrow pH gradient, it facilitates the precise migration and focusing of proteins at their respective pI positions, leading to well-resolved bands and improved accuracy in protein analysis.

High-resolution separation: The use of an ampholyte pH 5-6 in IEF enables high-resolution separation of proteins. The narrow pH range enhances the ability to discriminate proteins with similar pI values, resulting in sharper and well-defined protein bands. This enhances the sensitivity and specificity of protein detection, identification, and characterization.

Analysis of protein isoforms and modifications: Many proteins exist in multiple isoforms or undergo post-translational modifications, leading to variations in their pI values. Using an ampholyte pH 5-6 in IEF allows for the separation and identification of these protein isoforms and modified forms. This enables researchers to study protein heterogeneity, functional implications of isoforms, and the impact of modifications on protein behavior.

Biomarker discovery: IEF using an ampholyte pH 5-6 is valuable in biomarker discovery studies. By comparing protein expression profiles between different samples, potential biomarkers associated with specific diseases or conditions can be identified. The focused separation within the pH range of 5-6 allows for the detection of disease-specific protein alterations, aiding in diagnostic and prognostic applications.

Sample compatibility: IEF with an ampholyte pH 5-6 is compatible with various sample types, including complex biological samples, cell lysates, or purified protein preparations. This versatility allows for the analysis of proteins from different sources and simplifies the adaptation of IEF for different research applications.

Integration with other techniques: IEF using an ampholyte pH 5-6 can be combined with other techniques for comprehensive protein analysis. Following the separation in the first dimension, the resolved proteins can be further analyzed using techniques such as gel electrophoresis, Western blotting, or mass spectrometry, enabling a multi-dimensional approach for protein characterization.

Proteomics research: The use of an ampholyte pH 5-6 in IEF is valuable in proteomics research. It allows for the systematic analysis of protein expression patterns, modifications, and isoforms within the specific pH range. By studying proteins with pI values in the 5-6 range, researchers can gain insights into cellular processes, disease mechanisms, and protein-protein interactions.

In summary, using an ampholyte pH 5-6 in IEF offers focused separation, high-resolution analysis, characterization of protein isoforms and modifications, biomarker discovery potential, sample compatibility, integration with other techniques, and finds application in proteomics research and disease-related studies.