Ampholytes 2-4 (Servalyt 2-4)

Carrier ampholytes 2-4 by Serva

Servalyts 2-4 carrier ampholytes can be used in cIEF and gel IEF to identify acidic isotypes in highly purified protein samples such as monoclonal antibodies or other biosimilar products. This narrow range allows you to zoom in on the pH gradient across the entire gel. Ampholytes are supplied as a 40% sterile solution (0.2um filtered). They have a 3 year shelf life when stored at 4^oC unopened. Common working concentrations range from 3% to 5%. 


Benefits:

• Available in two sizes: 10ml and 25ml
• High resolution
• Fast staining and clear background
• Ready to use and blendable 

For comparative performance analysis see this article

Using an ampholyte pH 2-4 in isoelectric focusing (IEF) offers several benefits, including:

Specific separation of low pI proteins: The narrow pH range of 2-4 is well-suited for the separation of proteins with low isoelectric points (pI). It allows for a focused and precise separation of acidic proteins that have pI values within this range. This is particularly beneficial when analyzing samples enriched in acidic proteins or when targeting specific low pI proteins of interest.

High resolution for acidic proteins: The narrow pH range ensures a higher resolution for proteins with low pI values. The pH gradient established by the ampholytes enables these acidic proteins to migrate and focus precisely at their respective pI positions, resulting in sharp and well-resolved bands. This facilitates accurate identification and characterization of acidic proteins.

Minimization of interference: By using an ampholyte pH 2-4, the focusing range is limited to the acidic region, minimizing potential interference from proteins with higher pI values. This can be advantageous when dealing with complex samples containing a mixture of proteins with varying pI values, as it allows for selective separation and analysis of the acidic fraction.

Increased sensitivity for low pI proteins: The focused separation of low pI proteins achieved with an ampholyte pH 2-4 can enhance the sensitivity of detection methods. When analyzing low abundance acidic proteins, the reduced complexity of the pH range can lead to improved detection limits and a higher signal-to-noise ratio.

Simplified experimental setup: The use of a narrow pH range such as 2-4 simplifies the experimental setup for IEF. The preparation of the ampholyte mixture and the establishment of the pH gradient become more straightforward and manageable. This can save time and reduce potential sources of experimental variability.

Versatility with complementary techniques: IEF with an ampholyte pH 2-4 can be easily combined with other separation techniques. For example, after the acidic proteins have been separated using IEF, they can be further characterized or purified using additional techniques like gel electrophoresis, chromatography, or mass spectrometry. This integration of techniques allows for a comprehensive analysis of low pI proteins.

Research applications: The use of an ampholyte pH 2-4 in IEF finds particular relevance in research areas where low pI proteins play a crucial role, such as studying acidic enzymes, peptides, or specific protein isoforms. It provides a specialized tool for investigating these acidic components in complex biological samples.

In summary, using an ampholyte pH 2-4 in IEF offers specific separation and high resolution for low pI proteins, minimizes interference from other proteins, increases sensitivity, simplifies experimental setup, allows for versatility with complementary techniques, and finds application in research areas focused on acidic proteins.

"Serva ampholytes for High-Resolution Protein Focusing": Serva’s ampholytes offer researchers a reliable solution for achieving high-resolution protein focusing in IEF gels. With their broad pH range, enhanced resolution, and consistent performance, Serva ampholytes empower scientists to explore the intricate world of proteins with confidence.