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The IEF-SYS horizontal isoelectric focusing system is optimized for IEF applications using IPG Strips and precast horizontal IEF gels. The large 27 x 27cm ceramic cooling plate connects to an optional chiller for rapid and sustained cooling. The plates will accommodate two 125mm square or one 245 x 125mm precast gels, or a maximum of thirty 24cm IPG strips, at their respective operating temperatures of 4 and 20°C.
An acrylic electrode frame sits directly on the ceramic cooling plate for IEF gels, whereas a glass electrode frame may be employed when using IPG strips. In both applications, the positive and negative electrodes can be adjusted within the electrode frame - so that the voltage may be fine-tuned along each length of gel or IPG strip - and then held in position by a glass electrode weight. This unit has been proof tested with the following Serva Electrophoresis products: Serva IPG BlueStrips and Servalyt Precotes.
The IEF-SYS unit can be also adapted for wick-based electrophoresis with horizontal precast gels. The tank is a leak proof design with 99.99% Platinum electrodes.
Features include:
Complete System: IEF-SYS
Flat-bed isoelectric focusing unit includes running tank, lid, 1 x ceramic cooling plate, 1 x glass plate, 1 x electrode frame, 1 x anode electrode, 1 x cathode electrode, 1 x glass electrode weight, 2 x quick-fit tubes and 2 x 2mm power cables
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Technical Specifications: IEF-SYS |
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Maximum Sample Capacity |
30 IPG strips, 7cm to 24cm, Serva IPG BlueStrips |
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1 x 245 x 125mm or 2 x 125 x 125mm (W x L) SERVALYT™ PRECOTES™ IEF gels |
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Unit dimensions (W x D x H) |
46 x 41 x 11.5cm |
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Inner Tank Dimensions (W x D x H) |
37 x 31 x 8.5cm |
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Glass plate active dimensions (W x L x D) |
27 x 27 x 0.5cm |
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Electrode frame active dimensions (W x L x H) |
27 x 27 x 1cm |
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Cooling plate dimensions (W x L x D) |
27 x 27 x 3cm |
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Glass electrode weight active dimensions (W x L x H) |
20 x 26.5 x 1cm |
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Adjustable electrodes active dimensions (W x L x H) |
10 x 265 x 13mm |
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Recommended temperature for cooling plate 20°C during IEF with IPG strips |
20°C |
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Recommended temperature for cooling plate during IEF with IEF gels |
4°C |
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Power Output Connectors (diameter) |
Shrouded, 2mm |
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Recommended Power Supply |
Consort EV 232 or 262 |
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Recommended Running Conditions for 125mm long Servalyt Precote IEF gels |
Voltage Step |
1 |
2 |
3 |
4 |
5 |
End of run |
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Voltage |
300 |
600 |
1000 |
1200 |
1500 |
2000 |
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Time (min) |
20 |
20 |
30 |
30 |
30 |
60 |
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Volt-hours |
100 |
200 |
500 |
600 |
750 |
2000 |
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Recommended Running Conditions for 7cm SERVA IPG Strips |
Voltage Step |
1 |
2 |
3 |
4 |
5 |
End of run |
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Voltage |
150 |
300 |
600 |
1500 |
3000 |
330 |
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Time (min) |
0.5 |
0.5 |
0.5 |
0.5 |
2.5 |
<20 |
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Volt-hours |
75 |
150 |
300 |
750 |
7500 |
- |
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Recommended Running Conditions for 18cm SERVA IPG Strips |
Voltage Step |
1 |
2 |
3 |
4 |
End of Run |
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Voltage |
300 |
600 |
1500 |
3000 |
300 |
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Time (min) |
1 |
1 |
1 |
12.5 |
<20 |
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Volt-hours |
300 |
600 |
1500 |
37500 |
- |
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Snap-lock Connectors for Cooling Coil |
I.D. |
10mm |
O.D. |
12mm |
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Quick Fit Tubing |
I.D. |
10mm |
O.D. |
12mm |
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