Ampholytes 4-6 (Servalyt 4-6)

Carrier Ampholytes pH 4-6

SERVALYT 4-6 ampholytes are useful in identifying acidic isotypes in purified protein samples such as monoclonal antibodies or other biosimilar protein products.

Supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. Excellent for high resolution of acidic protein in isoelectric focusing (IEF), capillary isoelectric focusing (cIEF) and free flow electrophoresis applications.

Benefits:

• Available Two Sizes: 10 ml & 25ml
• High Resolution
• Widest Range Available
• Fast Staining and Clear Background
• Ready to Use or Blending
• 3 year shelf life

Comparison of different brands of carrier ampholytes

Using an ampholyte pH 4-6 in isoelectric focusing (IEF) offers several applications and benefits, including:

Enhanced separation of proteins with diverse pI values: The pH range of 4-6 allows for the separation of proteins with a wide range of isoelectric points (pI). It covers the intermediate acidic to slightly basic region, enabling the analysis of proteins with different pI values within this range. 

Increased resolution and selectivity: The use of an ampholyte pH 4-6 enables high-resolution separation of proteins with varying acidic and slightly basic characteristics. The pH gradient established by the ampholytes facilitates the migration and focusing of proteins at their respective pI positions, resulting in well-resolved and distinct protein bands. This enhances the accuracy of protein identification and characterization.

Improved separation of protein isoforms and post-translational modifications: Many proteins exhibit multiple isoforms or undergo post-translational modifications, resulting in variations in their pI values. Using an ampholyte pH 4-6 in IEF allows for the separation and identification of these protein isoforms and modified forms, providing insights into protein heterogeneity and its functional implications.

Compatibility with various sample types: IEF using an ampholyte pH 4-6 is compatible with various sample types, including biological fluids, cell lysates, or purified protein preparations. This versatility allows for the analysis of proteins from different sources and simplifies the adaptation of IEF for different research applications.

Biomarker discovery and disease research: The separation and analysis of proteins with an ampholyte pH 4-6 in IEF find relevance in biomarker discovery studies and disease-related research. By comparing protein expression patterns between different samples, potential biomarkers associated with specific diseases or physiological conditions can be identified. Additionally, the investigation of protein isoforms and modifications can contribute to a better understanding of disease mechanisms.

Integration with other techniques: IEF with an ampholyte pH 4-6 can be seamlessly integrated with other techniques for further protein analysis. After separation using IEF, the resolved proteins can be subjected to additional techniques such as gel electrophoresis, Western blotting, or mass spectrometry, enabling comprehensive protein characterization and identification.

Proteomics research: The use of an ampholyte pH 4-6 in IEF is valuable in proteomics research, allowing for the systematic analysis of protein expression patterns and modifications. It provides a means to compare protein profiles between different experimental conditions, study dynamic changes in protein expression, and investigate the roles of proteins with specific pI values.

In summary, using an ampholyte pH 4-6 in IEF offers enhanced separation, increased resolution and selectivity, improved separation of protein isoforms and modifications, compatibility with various sample types, relevance in biomarker discovery and disease research, integration with other techniques, and finds application in proteomics research.