Use a narrower pH range IPG Strip such as the IPG Strip pH 4-7 for acidic proteins and to gain greater resolution. The narrower the pH gradient range per strip the better the resolution. Very narrow or micro-range gradients allow for even greater resolution since there is more migration distance between proteins.
Standard, broad-range pH gradients (pH 3–10 linear and nonlinear strips) will reveal the greatest number of proteins, and will accommodate a larger sample volume. Use a non-linear gradient if you suspect a large number of proteins. In a non-linear broad range such as pH3-10, the middle pH range (pH 5-7) is more spread out than in linear ranges allowing for greater resolution.
SERVA IPG Strips are dried PAG gel strips containing an immobilized pH gradient for high resolution isoelectric focusing in 2D gel electrophoresis analysis of proteins. The polyacrylamide gel matrix is covalently bound to 3mm polyester GEL-FIX™ film for stability. Additionally, a non-binding cover film (GEL-FIX™ for Covers) protects the gel from damage and contamination. Each gel strip has its own lot number printed on and, therefore, is suitable for documentation according to GMP/GLP.
Gel Matrix: Polyacrylamide, 4%T, 3%C
<ul> <li>6KV, 150mA, 300W</li> <li>4 Parallel Outputs</li> <li>3 year warranty</li> </ul>
<ul> <li>Horizontal Isoelectric Focusing</li> <li>Economical, Robust</li> <li>Accomodates Precast horizontal IEF Gels & IPG strips</li> </ul>
<ul><li>Shortest Run Times</li> <li>Highest Voltage Available: 12KV</li> <li>Throughput: Six strips, 7 to 24 cm IPG Strips</li> </ul>
<p><span>A unique marker to calibrate 2D gels,or serve as internal LC/MS standard or added to protein samples</span></p> <p><span>Size: 1 kit</span></p>