Native Marker, Liquid Mix for BN/CN, Serva

Molecular Weight Marker for Blue and Clear Native PAGE. Ready-to-use. Contains native proteins ranging from 720 kDa to 21 kDa. 

Use for Native protein electrophoresis, a technique used to separate proteins based on their size, shape, and charge while maintaining their native (non-denatured) structure. Unlike SDS-PAGE, which denatures proteins, native electrophoresis preserves their functional conformation, allowing for the study of protein complexes and interactions. The most common forms include native PAGE (polyacrylamide gel electrophoresis) and agarose gel electrophoresis. The migration of proteins in the gel is influenced by their net charge and molecular weight, making this method useful for analyzing protein-protein interactions, enzymatic activity, and oligomeric states.

The Native marker contains the following proteins...

Blue Native (BN) and Clear Native (CN) electrophoresis are both techniques used to separate protein complexes while preserving their native state, but they differ in how they achieve this:

1. Blue Native (BN) Electrophoresis

   • Uses Coomassie Brilliant Blue G-250 to provide charge to the protein complexes.
   • The dye binds to proteins, imparting a negative charge, which allows migration in a charge-dependent manner.
   • Effective for resolving membrane protein complexes and other large protein assemblies.
   • Since the dye interacts hydrophobically with proteins, it can sometimes lead to minor structural alterations.

2. Clear Native (CN) Electrophoresis

   • Does not use Coomassie dye, keeping proteins in a truly detergent-stabilized native state.
   • Proteins migrate based on their intrinsic charge rather than a dye-induced charge.
   • Better suited for studying enzyme activities post-separation, as there is no interference from the dye.
   • Provides a more physiologically relevant separation but may result in lower resolution compared to BN-PAGE.

HS: 38220000
Storage Temperature: -15 °C to -25 °C