Alkaline Phosphatase  

This Alkaline phosphatase is from calf intestine and has 3000 U/mg or 60U/ul; made by Serva Electrophoresis

Economcal and optiimized  for the preparation of EIA-conjugates; no further dialysis necessary.

• Activity = 3000U/mg

• No dialysis required

• Ready to use

In 50 % glycerol, containing 5 mM Tris, 5 mM MgCl₂, 0.1 mM ZnCl₂, pH ca. 7.0.

Unit definition: 1 U catalyzes the hydrolysis of 1 µmole of 4-nitrophenyl phosphate per minute at 37 °C, pH 9.8 (DEA buffer).

Activity in other units: ca. 1100 U/mg at 25 °C, pH 9.6 (glycine buffer)

Substrates for Alkaline Phosphatase include:

• 4-Nitrophenyl phosphate·Na₂-salt (cat.no. 30770) Phenolphthalein diphosphate Na₄-salt (cat.no. 32050)
• 5-Bromo-4-chloro-3-indoxyl-phosphate-p-toluidine-salt (BCIP) (cat.no. 15247)
• 5-Bromo-4-chloro-3-indoxyl-phosphate Na₂-salt (cat.no. 15259) Naphthol-AS-BI-phosphate (cat.no. 29988)
• Naphthol-AS-MX-phosphate (cat.no. 30002) 1-Naphthyl phosphate Na-salt (cat.no. 30130)

DANGER:  Hazardous Goods

Storage Temperature: +2 °C to +8 °C