5-Bromo-4-chloro-3-indolyl-phosphate·p-toluidine-salt CAS 6578-06-9 Expand

5-Bromo-4-chloro-3-indolyl-phosphate·p-toluidine-salt CAS 6578-06-9, SERVA



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5-Bromo-4-chloro-3-indolyl-phosphate p-toluidine-salt

research grade



C8H6NO4BrClP·C7H9N or C15H15BrClN2O4PMr 433.6CAS 6578-06-9


Used in conjunction with NBT (cat. no. 30550) for detection of alkaline phosphatase.

Stock solution: 50 mg/ml in 100 % DMF. Store at 4 °C or -20 °C.

Staining solution for western blots: 66 µl NBT stock solution and 33 µl BCIP stock

solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)


Assay (HPLC)min. 99 %


EINECS: 229-506-1HS: 29339980

Storage Temperature: -15 °C to -25 °C