5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) CAS 7240-90-6, SERVA

5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal)

research grade

(X-Gal)

C₁₄H₁₅BrClNO₆ • M_r 408.6 • CAS 7240-90-6

X-gal is a well known histochemical substrate used to detect the β-galactosidase enzyme.

Identification of lac^f-colonies. X-gal is used to distinguish recombinant plasmids from parental

vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene.

Upon hydrolysis, X-gal yields a localized, insoluble blue precipitate making it exceptionally useful in

blotting, immunocytochemical, and ELISA assays. X-gal has been used for the detection of coliforms (E. coli)

in municipal water supplies and food products. X-gal is often used in conjunction with IPTG (Isopropyl-β-D-

thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing β-galactosidase activity.

Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants,

use in final concentration of 40 µg/ml.

EINECS: 230-640-8 • WGK: 1 • HS: 29389090

Storage Temperature: +2 °C to +8 °C