The SERVAGel IEF gels provide greater resolution, shelf life and better price value over competitive gels.
SERVAGel IEF gels are available in pH 3-10 and 4-7. By gel cassette design these gels provide a full 8cm resolving gel, thats 1cm or 14% more resolving gel than competitive gels. The combination of more resolving gel and use of broad IEF gels pH 3-10 gel and narrow IEF gels pH 4-7 provides a powerful solution to discover and monitor anibody isotypes and other proeins in research and Qa/QC applications.
The proprietary gel composition yeilds a stable gel with a 1 year shelf life which drastically reduces wastage and minimizes lot to lot variations, thus providing greater intra-lot gel to gel consistency.
IEF gels come in 5 and 10 gels packs with 10, 12 and 15 well configurations for optimal sample throughput. There is no need for special equipment and the gels fit most brands of electrophoresis chambers. IEF gel starter kits are available too.
Comparing price; SERVA IEF gels are $28.50/gel ($285/10 gels) vs $41.00/gel ($205/5 gels); a 30% savings.
Value and Versatility
We compared brand X and Y to the Servagel, all gave pretty much the same resolution; Serva seemed a bit cleaner. The reason I'm writing this is because we were also looking for value. The Servagel out shined the others; 10 gels for about the price of a 5 gel pack by the others. Plus I like the fact we can use these in the NEPHGE application to resolve the occasional troublesome basic proteins. Don R. QC.
We have used over 100 gels in the last year and are continuing to use them extensively. We prefer to make our own running buffers and have found that these gels work very well in the IEF mode. The 12-well gels that we prefer have 35 ul sample capacity which is great. The gels can be used in the NEPHGE mode. We have tried NEPHGE but don't use it because it is a non-equilibrium process. Among the 100-odd gels, we found only 3 gels with defective wells and we were very pleased that Biophoretics promptly issued credit for these gels. We are really pleased with the sales and service we have received from Biophoretics.
Our proteins were running basic, about pH 9. Thanks to your help we were able to resolve them using the Serva IEF gel and doing the NEPHGE protocol you gave us. Thanks again!