SERVA BluePrep Major Serum Potein Removal Kit, 25 reactions, SERVA
The SERVA BluePrep Major Serum Protein Removal Kit bases on spin-column chromatography and provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, alpha-antitrypsin, transferrin and haptoglobulin from serum and plasma samples. Process up to 10 samples in only 30 minutes. Based on ion exchange mechanism and not using specific antibodies, the kit can be used with serum samples from humans and various animals. The kit depletes 70 % of albumin, 90 % of alpha-antitrypsin, and 50 % of transferrin and haptoglobulin, enabling the visualization of low abundance proteins. The final elution is performed with choices of several different buffers suitable for: mass spectrometry, SDS-PAGe, 2 D-PAGE, microarrays.
Kit specifications:
- Max. protein input/spin column: 500 ug
- Min. protein input/spin column: 200 ug
- Min. elution volume: 100 ul (for max. protein recovery 200 µl)