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Protein Test Mixture 6 for SDS PAGE by SERVA Electrophoresis
Molecular weight markers for SDS gel electrophoresis. 7 protein markers, 6.5KDa - 97.4KDa, 10mg total lyophylized protein, use 5ul per lane.
Reconstitute with SDS sample buffer (125 mM Tris-HCl pH 6.8, 2 % SDS ,15 % glycerol, 10 mM DTT, 0.025 % bromophenol blue, 0.025 % Orange G) to final concentration of 1 mg/ml and apply 5 µl per lane when staining with SERVA Blue G, SERVA Blue R or Coomassie™.
For silver staining, e.g. using SERVA's Silver Staining Kit (cat.no. 39076), dilute 1:5 in 1x Laemmli buffer and apply 5 µl.
Standard proteins ranging from 6.5 kDa to 97.4 kDa:
Phosphorylase B Albumin bovine (BSA) Albumin egg Carbonic anhydrase Trypsin inhibitor (soybean) Cytochrome C Trypsin inhibitor (bovine lung) |
Mr 97 400 Mr 67 000 Mr 45 000 Mr 29 000 Mr 21 000 Mr 12 500 Mr 6 500 |
Coomassie = registered trademark of IC Ltd.
Storage Temperature: +2 °C to +8 °C
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