Salt Active Nuclease, SERVA Electrophoresis

Supplied as solution in 25 mM Tris-HCl pH 7.5, 5 mM MgCl2, 0.5 M NaCl, 0.01 % Triton, 50 % (v/v) glycerol.

Salt Active Nuclease is a highly active non-specific endonuclease from a marine bacterium that cleaves both DNA and RNA. It digests DNA versus RNA in a 10:1 ratio.

Salt Active Nuclease has optimum activity at 0.5 M NaCl, is active in the pH range of 7 to 10 and low temperatures, which makes the enzyme ideal for use in removal of nucleic acids from cell extracts and proteins samples. It will remove contaminating nucleic acids in a traditional protein buffer system. That guarantees the full protection of proteins while the nucleic acids are fully removed.

• Non specific endonuclease
• Optimum activity at high salt concentration (0.5 M NaCl)
• Active at low temperatures (20 % at 6 ºC)
• Broad pH range
• Temperature stable

Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute at 37 ºC, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25 ºC), 5 mM MgCl2, 500 mM NaCl.

HS: 35079090