Trypsin 1:250 from bovine pancreas, SERVA Electrophoresis
lyophil.
3.4.21.4 • CAS 9002-07-7
USP. Serin protease mixture suitable for cell culture. Contains chymotrypsin and non-proteolytic activities. Trypsin 1:250 is a special blend of enzymes designed to maintain maximum cell viability during gentle dissociation. Customary concentration for use: 250 mg/100 ml, pH 7 – 8.
Trypsin assay: 250 NF/USP U/mg
Total aerobic count plate none detectable
Moulds and yeasts <15 /g
Bacterial count Absent in 10 g material
(Salmonella, Staph. aureus, Ps. aeruginosa, Enterococcus sp.)
Unit definition: That amount of enzyme that causes hydrolysis of BAEE (benzoylarginine ethyl ester) such that the increase of light absorbance at 253 nm is 0.003 per minute.
EG-Index: 647-010-00-7 • EINECS: 232-650-8 • WGK: 1 • HS: 35079090
Storage Temperature: -15 °C to -25 °C
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