Nitro Blue tetrazolium chloride, CAS 298-83-9, Serva Expand

Nitro Blue tetrazolium chloride (NBT, Nitro BT), analytical grade, CAS 298-83-9, SERVA



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Nitro Blue tetrazolium chloride, Serva

analytical grade


(NBT, Nitro BT, Nitrotetrazolium Blue chloride, Ditetrazolium dye)

C40H30Cl2N10O6 • Mw 817.7 • CAS 298-83-9


A ditetrazolium compound for suitable for examining succinic dehydrogenase,

Glucose-6-phosphate dehydrogenase, and Xanthine oxidase activity. Used in

conjunction with BCIP for detection of alkaline phosphatase.


Stock solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C.


Staining solution for western blots: 66 µl NBT stock solution and 33 µl BCIP

stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)


Puritymin. 98.0 %


WARNING:  Hazardous Goods


Storage Temperature: +2 °C to +8 °C

EINECS: 206-067-4 • WGK: 1 • HS: 29339980