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Nitro Blue tetrazolium chloride
Analytical grade NBT greater than 98% purity, CAS # 298-83-9 by Serva
(NBT, Nitro BT, Nitrotetrazolium Blue chloride, Ditetrazolium dye)
C40H30Cl2N10O6 • Mw 817.7
NBT is a ditetrazolium compound for suitable for examining succinic dehydrogenase, Glucose-6-phosphate dehydrogenase, and Xanthine oxidase activity. Used in conjunction with BCIP for detection of alkaline phosphatase.
Stock solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C.
Staining solution for Western Blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)
Purity | min. 98.0 % |
WARNING: Hazardous Goods
Storage Temperature: +2 °C to +8 °C
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