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(Basic Blue 9, Methylene blue chloride, Methylthionine chloride)
C.I.52015 • C16H18N3S+·Cl- • Mr 319.86 •CAS [61-73-4]
Standardized according to DIN 58981. Methylene blue is very sensitive to oxidation
and usually contains demethylated products, called Azure A, B and C and thionine.
For staining and detecting RNA in PAGE (as 0.1 % solution in water), as a reversible
stain in Northern Blots, and as a water soluble nuclear staining dye, mostly used for
staining of blood cells.
λmax. (0.0001 % in water)
A 1 cm/λmax./0.0001 % in water
665 ± 2 nm
max. 15 %
max. 1.0 %
WARNING: Hazardous Goods
EINECS: 200-515-2 • WGK: 2L • HS: 32049000
Storage Temperature: +15 °C to +30 °C