Methylene Blue Expand

Methylene Blue, CAS 61-73-4, SERVA



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Methylene Blue


(Basic Blue 9, Methylene blue chloride, Methylthionine chloride)

C.I.52015 • C16H18N3S+·Cl- • Mr 319.86 •CAS [61-73-4]


Standardized according to DIN 58981. Methylene blue is very sensitive to oxidation

and usually contains demethylated products, called Azure A, B and C and thionine.

For staining and detecting RNA in PAGE (as 0.1 % solution in water), as a reversible

stain in Northern Blots, and as a water soluble nuclear staining dye, mostly used for

staining of blood cells.


λmax. (0.0001 % in water)

A 1 cm/λmax./0.0001 % in water

Water (KF)

Sulfated ash

665 ± 2 nm

min. 0.17

max. 15 %

max. 1.0 %


WARNING:  Hazardous Goods


EINECS: 200-515-2 • WGK: 2L • HS: 32049000
Storage Temperature: +15 °C to +30 °C

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