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Nitro Blue tetrazolium chloride (NBT)
CAS # 298-83-9
(Nitroblue tretrazolium chloride, Nitrotetrazolium Blue chloride, Ditetrazolium dye)
Molecular formula: C40H30Cl2N10O6, Mw 817.7
NBT Purity is greater than 98%
Nitroblue tetrazolium chloride (NBT) is a water soluble enzyme substrate that forms a light yellowish color in solution before reacting. It is used in the detection of alkaline phosphatase along with BCIP (5-Bromo-4-chloro-3-indolyl phosphate). With BCIP as the alkaline phosphate substrate, NBT is the oxidant in the reaction to yield a dark blue color.
Light sensitive
Store in a dry, dark location between 2 and 8C
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