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Based on the bichinchoninic acid method (1), absorbance is directly proportional to protein concentration. Proteins reduce alkaline Cu(II) to Cu(I). Bichinchoninic acid from a purple complex with Cu(I) with an absorbance maximum at 562 nm.
Smith, P.K., et al. (1985) Anal. Biochem. 150, 76 – 85
Storage Temperature: +2 °C to +8 °C