Nitro Blue tetrazolium chloride, CAS 298-83-9, Serva

Nitro Blue tetrazolium chloride (NBT, Nitro BT), analytical grade, CAS 298-83-9, SERVA

30550.02
$126.66

Nitro Blue tetrazolium chloride 

  • NBT
  • CAS 298-83-9
 

Nitro Blue tetrazolium chloride 

Analytical grade NBT greater than 98% purity, CAS # 298-83-9 by Serva

 

(NBT, Nitro BT, Nitrotetrazolium Blue chloride, Ditetrazolium dye)

C40H30Cl2N10O6 • Mw 817.7 

 

NBT is a ditetrazolium compound for suitable for examining succinic dehydrogenase, Glucose-6-phosphate dehydrogenase, and Xanthine oxidase activity. Used in conjunction with BCIP for detection of alkaline phosphatase.

Stock solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C.

Staining solution for Western Blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)

 

Purity min. 98.0 %

 

WARNING:  Hazardous Goods

 

Storage Temperature: +2 °C to +8 °C

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