Nitro Blue tetrazolium chloride, Serva
(NBT, Nitro BT, Nitrotetrazolium Blue chloride, Ditetrazolium dye)
C40H30Cl2N10O6 • Mw 817.7 • CAS 298-83-9
A ditetrazolium compound for suitable for examining succinic dehydrogenase,
Glucose-6-phosphate dehydrogenase, and Xanthine oxidase activity. Used in
conjunction with BCIP for detection of alkaline phosphatase.
Stock solution: 50 mg/ml in 70 % DMF. Store at 4 °C or -20 °C.
Staining solution for western blots: 66 µl NBT stock solution and 33 µl BCIP
stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)
WARNING: Hazardous Goods
Storage Temperature: +2 °C to +8 °C
EINECS: 206-067-4 • WGK: 1 • HS: 29339980