5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) CAS 7240-90-6 Expand

5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) CAS 7240-90-6, SERVA



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5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal)

research grade



C14H15BrClNO6Mr 408.6CAS 7240-90-6


X-gal is a well known histochemical substrate used to detect the β-galactosidase enzyme.

Identification of lacf-colonies. X-gal is used to distinguish recombinant plasmids from parental

vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene.

Upon hydrolysis, X-gal yields a localized, insoluble blue precipitate making it exceptionally useful in

blotting, immunocytochemical, and ELISA assays. X-gal has been used for the detection of coliforms (E. coli)

in municipal water supplies and food products. X-gal is often used in conjunction with IPTG (Isopropyl-β-D-

thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing β-galactosidase activity.

Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants,

use in final concentration of 40 µg/ml.


Assay (HPLC)min. 99 %


EINECS: 230-640-8WGK: 1HS: 29389090

Storage Temperature: +2 °C to +8 °C