SERVALYT™ 3 - 7, Carrier Ampholytes pH 3 - 7
SERVALYT™ 3 - 7, Carrier Ampholytes pH 3 - 7
SERVALYT™ 3 - 7, Carrier Ampholytes pH 3 - 7
SERVALYT™ 3 - 7, Carrier Ampholytes pH 3 - 7

Ampholytes 3-7, Servalyts

42945.01
$240.65

Ampholyte 3-7 (Servalyt 3-7)

  • Servalytes Provide Higher Resolution
  • Cleaner Separations
  • Clearer Backgrounds
  • Two Sizes Available: 10ml & 25ml
Volumes

 

Ampholytes 3–7 (Servalyt 3-7)

Carrier ampholytes 3-7

SERVALYT™ 3-7 ampholytes are useful in gel IEF and cIEF for identifying acidic isotypes in purified protein samples such as monoclonal antibodies or other biosimilar protein products.

Supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. Excellent for isoelectric focusing (IEF), capillary isoelectric focusing (cIEF) and free flow electrophoresis.

 

Benefits:

  • Available Two Sizes: 10 ml & 25ml
  • High Resolution
  • Widest Range Available
  • Fast Staining and Clear Background
  • Ready to Use or Blending
  • 3 year shelf life



Instructions for Servalyt carrier ampholytes

Comparison of different brands of carrier ampholytes

Using an ampholyte pH 3-7 in isoelectric focusing (IEF) offers several applications and benefits, including:

Broad pH range coverage: The pH range of 3-7 provides a wide coverage of isoelectric points (pI), allowing for the separation of proteins with diverse pI values. It enables the analysis of both acidic and slightly basic proteins, making it suitable for studying a broad range of proteins and their isoforms.

High-resolution separation: The use of an ampholyte pH 3-7 enables high-resolution separation of proteins with varying pI values. The pH gradient established by the ampholytes facilitates the migration and focusing of proteins at their respective pI positions, resulting in well-resolved and distinct bands. This enhances the accuracy of protein identification and characterization.

Versatile protein analysis: The ampholyte pH 3-7 offers versatility in protein analysis. It can be applied to various sample types, including biological fluids, cell lysates, or purified protein preparations. This flexibility allows researchers to study proteins from different sources and adapt IEF to different research applications.

Identification of protein variants and modifications: Many proteins exhibit multiple isoforms or undergo post-translational modifications, resulting in different pI values. Using an ampholyte pH 3-7 in IEF facilitates the separation and identification of these protein variants and modifications, providing insights into protein heterogeneity and its functional implications.

Complementary to other separation techniques: IEF using an ampholyte pH 3-7 can be combined with other separation techniques to enhance protein analysis. For example, after the separation of proteins using IEF, further characterization can be performed using techniques like gel electrophoresis, Western blotting, or mass spectrometry, enabling comprehensive protein analysis. 

Biomarker discovery: IEF with an ampholyte pH 3-7 is valuable in biomarker discovery studies. By separating proteins based on their pI values, it allows for the identification of potential biomarkers associated with specific diseases or physiological conditions. This can have important implications in diagnostic and therapeutic applications.

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