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SERVALYT™ ampholytes pH 4.2-4.9 are supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf life is up to 3 years. The common working concentration is in the range of 3 % to 5 %.
These can be used for capillary isoelectric focusing (cIEF), or casting horizontal IEF gels or tube gels. Gels can be prepared in glass tubes or in horizontal gel format. SERVALYT™ ampholytes are recommended for rehydration of dry IPG gel strips in 2DGE, DIGE, e.g. SERVA IPG BlueStrips, and medium-free IEF systems, e.g. free flow and capillary electrophoresis and cIEF.