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A 96-well plate format assay kit for protein quantification, based on protein precipitation insoluble dye complexes with acidic, methanolic amido black 10B solution (1, 2). After precipitation the protein-dye complexes are spun down. The pellet is washed and resolubilized. The thereby released dye amount is measured at 620 nm. Linearity from 100 to 1750 µg/ml protein. In triple measurement 23 samples can be analyzed (plus BSA standard and blank).
Note: Ampholytes bind to the dye as well and therefore simulate too high protein concentrations. Protein measurement of analytic solutions for 2DGE must be done before ampholyte (Servalyte) addition.
The kit contains 10x dye concentrate, elution solution, bovine serum albumin as standard reference, 96-well plates