IEF-SYS
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The IEF-SYS horizontal isoelectric focusing system is optimized for IEF applications using IPG Strips and precast horizontal IEF gels. The large 27 x 27cm ceramic cooling plate connects to an optional chiller for rapid and sustained cooling. The plates will accommodate two 125mm square or one 245 x 125mm precast gels, or a maximum of thirty 24cm IPG strips, at their respective operating temperatures of 4 and 20°C.
An acrylic electrode frame sits directly on the ceramic cooling plate for IEF gels, whereas a glass electrode frame may be employed when using IPG strips. In both applications, the positive and negative electrodes can be adjusted within the electrode frame - so that the voltage may be fine-tuned along each length of gel or IPG strip - and then held in position by a glass electrode weight. This unit has been proof tested with the following Serva Electrophoresis products: Serva IPG BlueStrips and Servalyt Precotes.
The IEF-SYS unit can be also adapted for wick-based electrophoresis with horizontal precast gels. The tank is a leak proof design with 99.99% Platinum electrodes.
Features include:
Complete System: IEF-SYS
Flat-bed isoelectric focusing unit includes running tank, lid, 1 x ceramic cooling plate, 1 x glass plate, 1 x electrode frame, 1 x anode electrode, 1 x cathode electrode, 1 x glass electrode weight, 2 x quick-fit tubes and 2 x 2mm power cables
Technical Specifications: IEF-SYS | |||||||||
Maximum Sample Capacity | 30 IPG strips, 7cm to 24cm, Serva IPG BlueStrips | ||||||||
1 x 245 x 125mm or 2 x 125 x 125mm (W x L) SERVALYT™ PRECOTES™ IEF gels | |||||||||
Unit dimensions (W x D x H) | 46 x 41 x 11.5cm | ||||||||
Inner Tank Dimensions (W x D x H) | 37 x 31 x 8.5cm | ||||||||
Glass plate active dimensions (W x L x D) | 27 x 27 x 0.5cm | ||||||||
Electrode frame active dimensions (W x L x H) | 27 x 27 x 1cm | ||||||||
Cooling plate dimensions (W x L x D) | 27 x 27 x 3cm | ||||||||
Glass electrode weight active dimensions (W x L x H) | 20 x 26.5 x 1cm | ||||||||
Adjustable electrodes active dimensions (W x L x H) | 10 x 265 x 13mm | ||||||||
Recommended temperature for cooling plate 20°C during IEF with IPG strips | 20°C | ||||||||
Recommended temperature for cooling plate during IEF with IEF gels | 4°C | ||||||||
Power Output Connectors (diameter) | Shrouded, 2mm | ||||||||
Recommended Power Supply | Consort EV 232 or 262 | ||||||||
Recommended Running Conditions for 125mm long Servalyt Precote IEF gels | Voltage Step | 1 | 2 | 3 | 4 | 5 | End of run | ||
Voltage | 300 | 600 | 1000 | 1200 | 1500 | 2000 | |||
Time (min) | 20 | 20 | 30 | 30 | 30 | 60 | |||
Volt-hours | 100 | 200 | 500 | 600 | 750 | 2000 | |||
Recommended Running Conditions for 7cm SERVA IPG Strips | Voltage Step | 1 | 2 | 3 | 4 | 5 | End of run | ||
Voltage | 150 | 300 | 600 | 1500 | 3000 | 330 | |||
Time (min) | 0.5 | 0.5 | 0.5 | 0.5 | 2.5 | <20 | |||
Volt-hours | 75 | 150 | 300 | 750 | 7500 | - | |||
Recommended Running Conditions for 18cm SERVA IPG Strips | Voltage Step | 1 | 2 | 3 | 4 | End of Run | |||
Voltage | 300 | 600 | 1500 | 3000 | 300 |
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Time (min) | 1 | 1 | 1 | 12.5 | <20 |
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Volt-hours | 300 | 600 | 1500 | 37500 | - |
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Snap-lock Connectors for Cooling Coil | I.D. | 10mm | O.D. | 12mm | |||||
Quick Fit Tubing | I.D. | 10mm | O.D. | 12mm |
<ul> <li>6KV, 150mA, 300W</li> <li>4 Parallel Outputs</li> <li>3 year warranty</li> </ul>
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">Dimensions: 125 x 125mm x 150um </span></li> <li>Made with Servalyt Ampholytes</li> <li>Easy to Handle Precast IEF Horizontal Gel on gel Backing</li> </ul>
1 rating(s) |
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">Dimensions: 245 x 125mm x 150um</span></li> <li>Made with Servalyt Ampholytes</li> <li>Easy to Handle Precast IEF Horizontal Gel on gel Backing</li> </ul>
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">Dimensions: 125 x 125mm x 150um</span></li> <li>Made with Servalyt Ampholytes</li> <li>Easy to Handle Precast IEF Horizontal Gel on gel Backing</li> </ul>
<ul> <li>Dimensions: 245 x 125mm x 150um</li> <li>Made with Servalyt Ampholytes</li> <li>Easy to Handle Precast IEF Horizontal Gel on gel backing</li> </ul>
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">Dimensions: 125 x 125mm x 300um</span></li> <li>Made with Servalyt Ampholytes</li> <li>Easy to Handle Precast IEF Horizontal Gel on gel backing</li> </ul>
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">Dimensions: 125 x 125mm x 150um</span></li> <li>Made with Servalyt Ampholytes</li> <li>Easy to Handle Precast IEF Horizontal Gel on gel Backing</li> </ul>
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">Use for Western Blotting & IEF</span></li> <li>Available in .30mm Gel Layer</li> <li>Precast on Porous Mesh Backing</li> </ul>
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">No pH Gradient</span></li> <li>Custom Make the Gradient - best resolution</li> <li>Use for Western Blotting & IEF</li> </ul>
<ul> <li><span style="font-size: 12px; line-height: 1.5em;">No pH Gradient</span></li> <li>Custom Make the Gradient You Need - best resolution</li> <li>Available in .30mm Thick Gel Layer</li> </ul>
<ul> <li><span style="line-height: 1.5em;">IEF Starter for Horizontal IEF <br /></span></li> <li><span style="line-height: 1.5em;">3 PRECOTE GELS, pH 3-10</span></li> <li>Format: 125 x 125 mm, .30mm</li> </ul>
1 rating(s) |
<ul><li>Available Lenths: 7cm, 11cm, 17cm, 18cm 24cm</li> <li>Individually Lot Numbered : GMP/GLP Conformity</li> <li>Reliable, Consistent Performance</li> </ul>
<ul> <li><span>Easy-to-use</span></li> <li><span>High Sensitivity</span></li> <li><span>Reproducible results</span></li> </ul>
<ul> <li><span>Ready to use.</span></li> <li><span>9 proteins, 13 isoforms</span></li> <li><span>Size: 500 ul</span></li> </ul>
1 rating(s) |