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Macerozyme R-10

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Macerozyme R-1


Pectinase


CAS # 9032-75-1


Macerozyme R-10
is  an enzyme used to aid the breakdown of plant tissue to create single cell isolates. It is often used in conjuction with  Onozuka R-10 (ref. B16419) or with  Onuzuka RS (ref. B16420).

 

Activities: Pectinase apx. 0.5 U/mg

 

Unit definition: 1 U catalyzes the liberation of 1 µmole of reducing groups from pectic acid per minute at 25 °C, pH 4.5 calculated as galacturonic acid. Reducing groups determined with alkaline copper reagent.

Hemicellulase: apx. 0.25 U/mg

Unit definition: 1 U is equivalent to 1 µmole of reducing groups released from beechwood xylan per hour at 37 °C, pH 5.5, calculated as xylose.

Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent.

  • Optimum pH: 3.5 - 7.0
  • Optimum temperature: 40 - 50 °C.

 

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