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Onozuka RS is a multi-component enzyme system with three times greater xylanase activity that other celulluases (Cellulase R-10). Xylanases are hydrolytic enzymes that randomly cleave the beta 1,4 backbone of the plant cell wall polysaccharide xylan.
Although the preparation has high cellulase activity, it also contains hemicellulases, and degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Widely used for the isolation of protoplasts and often with Macerozyme R-10.
Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent.
Extraneous activities: Contains α-amylase, pectinase, protease and hemicellulase.