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Onozuka R-10 is multi-component enzyme system from Trichoderma viride used to degrade plant cell walls and isolate protoplasts. It is often used in combination with Macerozyme R-10. This preparation has a high cellulase activity, contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides.
Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent.
Extraneous activities: α-amylase ca. 0.8 U, pectinase ca. 0.4 U, protease ca. 0.01 DMC-U, hemicellulase ca. 1 U/mg (1 U catalyzes the liberation of 1 µmole reducing groups from xylan per hour at 37 °C, pH 5.5, calculated as xylose).