This Alkaline phosphatase is from calf intestine and has 3000 U/mg or 60U/ul; made by Serva Electrophoresis
Economcal and optiimized for the preparation of EIA-conjugates; no further dialysis necessary.
Activity = 3000U/mg
No dialysis required
Ready to use
In 50 % glycerol, containing 5 mM Tris, 5 mM MgCl2, 0.1 mM ZnCl2, pH ca. 7.0.
Unit definition: 1 U catalyzes the hydrolysis of 1 µmole of 4-nitrophenyl phosphate per minute at 37 °C, pH 9.8 (DEA buffer).
Activity in other units: ca. 1100 U/mg at 25 °C, pH 9.6 (glycine buffer)
Substrates for Alkaline Phosphatase include:
- 4-Nitrophenyl phosphate·Na2-salt (cat.no. 30770) Phenolphthalein diphosphate Na4-salt (cat.no. 32050)
- 5-Bromo-4-chloro-3-indoxyl-phosphate-p-toluidine-salt (BCIP) (cat.no. 15247)
- 5-Bromo-4-chloro-3-indoxyl-phosphate Na2-salt (cat.no. 15259) Naphthol-AS-BI-phosphate (cat.no. 29988)
- Naphthol-AS-MX-phosphate (cat.no. 30002) 1-Naphthyl phosphate Na-salt (cat.no. 30130)
DANGER: Hazardous Goods
Storage Temperature: +2 °C to +8 °C