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Supplied as solution in 50 mM Tris-HCl pH 8.0, 5 mM MgSO4, 50 % (v/v) glycerol. Cyanase is a cloned highly active non-Serratia based non-specific endonuclease that degrades single and double stranded DNA and RNA in as little as 1 minute.
The enzyme is active over a broad pH range from 6 to 10, a wide temperature range up to 50 °C and NaCl concentration up to 100 mM. Cyanase is fully active in DTT up to 100 mM, and most common non-ionic detergents up to 1 % including Triton X-100, Tween 20. It is active in Urea up to 3 M and is unaffected by chemical lysate treatments such as lysozyme or detergent.
Cyanase is easily removed from samples after the reaction is finished using the Cyanase inactivation resin. The resin can be easily filtered or spun down to remove from the sample removing the Cyanase with it.
Because of its unique properties, Cyanase is effective for cell lysate clearance, protein and viral purification and nucleic acid removal from samples.
Unit definition: One Unit is the amount of enzyme that degrades 3 µg of full length λ DNA completely at 37 °C in 1 minute in 50 mM Tris-HCl pH 8.0, 6 mM MnSO4.
Cyanase is a registered trademark of RiboSolutions, Inc.
Storage Temperature: -15 °C to -25 °C