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5-Bromo-4-chloro-3-indolyl-phosphate•p-toluidine-salt, CAS [6578-06-9], Serva

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5-Bromo-4-chloro-3-indolyl-phosphate·p-toluidine-salt, Serva

research grade (BCIP)

 

C8H6NO4BrClP·C7H9N or C15H15BrClN2O4P • MW 433.6 • CAS [6578-06-9]

 

Used in conjunction with NBT (cat. no. 30550) for detection of alkaline phosphatase.

Stock solution: 50 mg/ml in 100 % DMF. Store at 4 °C or -20 °C.

Staining solution for western blots: 66 µl NBT stock solution and 33 µl BCIP stock solution in 10 ml staining buffer (100 mM NaCl, 5 mM MgCl2, 100 mM Tris; pH 9.5)

 

Assay (HPLC)   min. 99 %

 

Storage Temperature: -15 °C to -25 °C

EINECS: 229-506-1 • HS: 29339980

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