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A thermo-conductive plate holder for 96-well u-bottom SBS-format plates. The top surface of the CoolSink has 96 u-shaped wells to accommodate the under-surface of the u-bottom plate so each well nests in maximum contact with the CoolSink alloy. When placed on ice, dry ice, liquid nitrogen, water bath or any other temperature source, CoolSink 96U provides rapid and evenly-distributed cooling or heating to all wells, eliminating "edge effect."