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35 g for 1 liter medium
For cultivation of E. coli in molecular biology.
10 g/l Tryptone
5 g/l Yeast extract
5 g/l NaCl
15 g/l Agar
For making 1 L liquid medium suspend 35 g in 900 ml distilled water, adjust the pH to 7.0
with approximately 0.2 ml of 5N NaOH, fill up to a final volume of 1 L with deionized water
and sterilize by autoclaving. Cool to 45 °C prior to dispensing into sterile petri dishes.
Storage Temperature: +15 °C to +30 °C