SERVALYT™ 2 - 9T, Carrier Ampholytes pH 2-9T

Ampholytes 2-9 Seed Mix, Servalyts

42935.01
$240.65

Ampholytes 2-9 Seed Mix (Servalyt 2-9)

  • Servalytes Provide Sharper Focuing
  • Higher Resolution
  • Cleaner Separation
  • Three Sizes Available: 10ml, 25ml & 100ml

 

Volumes

 

Ampholytes 2-9 seed mix (Servalyt 2-9) 

Carrier Ampholytes pH 2-9 Seed Mix

Servalyt ampholytes are used for IEF gels, capillary electrophoresis (cIEF) and free-flow electrophoresis 


SERVALYT™ 2-9 "Seed Mix" ampholytes can be used to identify seed purity. All ampholytes are supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. 

 

Benefits:

  • Available Two Sizes: 10 ml & 25ml
  • High Resolution
  • Widest Range Available
  • Fast Staining and Clear Background
  • Ready to Use or Blending
  • 3 year shelf life



Instructions for Servalyt ampholyte

Comparative analysis of different brands of ampholytes

Using an ampholyte pH 2-9 SEED MIX in isoelectric focusing (IEF) can offer several benefits, including:

Expanded pH range: The pH 2-9 SEED MIX covers a broad range of pH values, allowing for the separation of proteins with various isoelectric points (pI). This expanded range increases the versatility and resolution of IEF, enabling the separation of proteins across a wider spectrum.

Enhanced resolution and selectivity: The wider pH range provided by the ampholyte pH 2-9 SEED MIX allows for better resolution and selectivity in the separation of proteins. This is especially advantageous when working with complex samples or mixtures containing proteins with diverse pI values. It enables the differentiation of closely related proteins and improves the overall separation quality.

Precise focusing of proteins: The ampholyte pH 2-9 SEED MIX facilitates efficient and accurate focusing of proteins during IEF. The pH gradient established by the ampholytes ensures that each protein migrates towards its specific pI position, resulting in sharp and well-defined bands. This promotes optimal separation and prevents smearing or overlapping of protein bands.

Compatibility with various detection methods: IEF using an ampholyte pH 2-9 SEED MIX is compatible with different detection methods commonly employed in protein analysis, such as staining, immunoblotting, or mass spectrometry. This allows for further characterization, identification, and quantification of proteins based on their migration pattern or specific properties 

Reproducibility and consistency: The use of a standardized ampholyte pH 2-9 SEED MIX helps ensure reproducible and consistent results in IEF experiments. The pre-made mixtures provide a stable pH gradient that remains consistent across multiple runs, allowing for reliable and comparable data analysis.

Scalability and applicability: IEF with an ampholyte pH 2-9 SEED MIX can be easily scaled up for large-scale protein separations, making it suitable for various research, industrial, or clinical applications that require high-throughput analysis of protein samples.

In summary, using an ampholyte pH 2-9 SEED MIX in IEF offers an expanded pH range, enhanced resolution and selectivity, precise protein focusing, compatibility with various detection methods, reproducibility, and scalability. These benefits contribute to improved protein separation, analysis, and characterization in diverse fields of study.

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