SERVALYT™ ampholytes are supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. Technical grade SERVALYT™ carrier ampholyte pH 4-9 T (Cat. No. 42910) is economical to use if preparative work is envisaged. The T grade SERVALYT™ is not sterile filtered. Gels can be prepared in glass tubes or in horizontal gel format (the IEF gel is cut into strips applied to the second dimension (SDS PAGE). SERVALYT™ ampholytes are recommended for rehydration of dry IPG gel strips in 2DGE, DIGE, e.g. SERVA IPG BlueStrips, and medium-free IEF systems, e.g. free flow and capillary electrophoresis. SERVALYT™ 3-10 IsoDalt (Cat. No. 42951) is particularly suited to 2D-electrophoresis.