Ampholytes 3-4 (Servalyt 3-4)

Carrier ampholytes 3-4 by Serva

SERVALYT™ 3-4 carrier ampholytes are useful in gel IEF and cIEF for identifying acidic isotypes in purified protein samples such as monoclonal antibodies or other biosimilar protein products. Ampholyes are supplied as 40 % aqueous concentrate and sterile filtered (0.2 μm). If stored at 4 °C and unopened shelf is up to 3 years. The common working concentration is in the range of 3 % to 5 %. Excellent for high resolution of acidic proteins in isoelectric focusing(IEF), capillary electrophoresis(cIEF) and free flow electrophoresis.

Benefits:

• Available Two Sizes: 10 ml & 25ml
• High Resolution
• Widest Range Available
• Fast Staining and Clear Background
• Ready to Use or Blending
• 3 year shelf life

Ampholyte instructions

Comparative analysis of different brands of ampholytes

Using an ampholyte pH 3-4 in isoelectric focusing (IEF) offers several benefits, including: 

Specific separation of proteins with low pI: The narrow pH range of 3-4 is well-suited for the separation of proteins with low isoelectric points (pI). It allows for focused separation and resolution of acidic proteins that have pI values within this range. This is particularly advantageous when analyzing samples enriched in acidic proteins or when targeting specific low pI proteins of interest.

High resolution for low pI proteins: The use of an ampholyte pH 3-4 enables high-resolution separation of proteins with low pI values. The narrow pH gradient created by the ampholytes allows for precise migration and focusing of acidic proteins at their respective pI positions. This results in sharp and well-resolved protein bands, facilitating accurate identification and characterization.

Minimization of interference: By using an ampholyte pH 3-4, the focusing range is limited to the acidic region, minimizing potential interference from proteins with higher pI values. This is particularly beneficial when dealing with complex samples containing a mixture of proteins with varying pI values, as it allows for selective separation and analysis of the low pI acidic fraction.

Enhanced sensitivity for low pI proteins: The focused separation of low pI proteins achieved with an ampholyte pH 3-4 can enhance the sensitivity of detection methods. When analyzing low abundance acidic proteins, the reduced complexity of the pH range can lead to improved detection limits and a higher signal-to-noise ratio.

Simplified experimental setup: The use of a narrow pH range such as 3-4 simplifies the experimental setup for IEF. The preparation of the ampholyte mixture and the establishment of the pH gradient become more straightforward and manageable. This saves time and reduces potential sources of experimental variability.

Research applications: Using an ampholyte pH 3-4 in IEF finds particular relevance in research areas where low pI proteins play a crucial role, such as studying acidic enzymes, peptides, or specific protein isoforms. It provides a specialized tool for investigating these low pI acidic components in complex biological samples.

In summary, using an ampholyte pH 3-4 in IEF offers specific separation and high resolution for low pI proteins, minimizes interference from other proteins, increases sensitivity, simplifies experimental setup, and finds application in research areas focused on acidic proteins.